Journal: Oncology reports

Article Title: Gonadotropins promote human ovarian cancer cell migration and invasion via a cyclooxygenase 2-dependent pathway.

doi: 10.3892/or.2017.5784

Figure Lengend Snippet: Figure 1. FSHR and LHR were expressed in SKOV3 and HO8910 cells but did not affect cell proliferation and apoptosis. Expression of FSHR and LHR was examined by immunocytochemical staining (A), RT-PCR (B), and western blotting (C). HO8910 and SKOV3 cells were treated with FSH and LH (0, 100, and 500 mIU/ml) alone or in combination, but no changes in proliferation (D and E) and apoptosis (F) were observed.

Article Snippet: The cells were then incubated with FSHR antibody (cat. PB1120; 1:200, Boster) or LHR antibody (cat. A01120; 1:500, Boster) at 4 ̊C overnight.

Techniques: Expressing, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Plant Signaling & Behavior

Article Title: Proteome-wide analysis reveals G protein-coupled receptor-like proteins in rice ( Oryza sativa )

doi: 10.1080/15592324.2024.2365572

Figure Lengend Snippet: Prediction of superfamily and family of candidate OsGPCRLPs.

Article Snippet: InterPro identified OsGPCRLP930 as a member of the GCR1-cAMP receptor family (IPR022343: InterPro family) with GCR1-cAMP receptor (PR02001: PRINTS) conserved motifs (32–50, 51–66, 82–100, 223–241 and 255–274 aa) and GCR1 family (IPR022340: InterPro family) with putative plant GPCR and GCR1 (PR02000: PRINTS) signature motifs (21–36, 38–52, 54–76, 84–97, 99–117, 122–137, 161–173, 174–197, 217–238, 247–264 and 265–281 aa).

Techniques:

Journal: Molecular Pharmacology

Article Title: Discovery and Characterization of Novel GPR39 Agonists Allosterically Modulated by Zinc

doi: 10.1124/mol.116.106112

Figure Lengend Snippet: Concentration-dependent and GPR39-dependent cAMP (Gs) responses, as measured by the Glosensor assay, to GPR39-C3 (A), LY2784544 (B), or GSK2636771 (C) with various concentrations of Zn2+. Responses in the absence of Zn2+ are shown in (D), (E), and (F). Responses to ZnCl2, NiCl2, and NiSO4 are shown in (G) and (H) and (I), respectively. The results are the mean ± S.E.M. of at least three independent experiments performed in quadruplicate.

Article Snippet: Receptor-mediated Gs pathway signaling was measured using a split-luciferase receptor assay (GloSensor cAMP assay; Promega, Madison, WI).

Techniques: Concentration Assay

Journal: Molecular Pharmacology

Article Title: Discovery and Characterization of Novel GPR39 Agonists Allosterically Modulated by Zinc

doi: 10.1124/mol.116.106112

Figure Lengend Snippet: Concentration-dependent cAMP production (Gs pathway) to GPR39-C3 (A), LY2784544 (B), and GSK2636771 (C) in the presence and absence of 100 μM Zn2+ at wild-type (WT) and H17A/H19A mutant receptors as measured by the Glosensor assay. Concentration-dependent cAMP production (Gs pathway) to ZnCl2 (D), NiCl2 (E), or NiSO4 (F) at wild-type or H17A/H19A mutant receptors as measured by the Glosensor assay. The results are the mean ± S.E.M. of at least eight independent experiments, each in quadruplicate.

Article Snippet: Receptor-mediated Gs pathway signaling was measured using a split-luciferase receptor assay (GloSensor cAMP assay; Promega, Madison, WI).

Techniques: Concentration Assay, Mutagenesis